The Mycology Reference Laboratory-CNM, a group that belongs to REIPI, recently published the following method in the AAC journal: High-Resolution Melting assay for genotyping variants of the CYP2C19 enzyme and predicting Voriconazole effectiveness

The Mycology Reference Laboratory-CNM, a group that belongs to REIPI, recently published the following method in the AAC journal: High-Resolution Melting assay for genotyping variants of the CYP2C19 enzyme and predicting Voriconazole effectiveness Voriconazole is a triazole antifungal agent recommended as primary treatment for invasive aspergillosis, as well as some other mold infections. However, it…

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Characterization of carbapenemase-producing Klebsiella oxytoca in Spain, 2016-2017

Summary. The increase of multidrug-resistant carbapenemase-producing Enterobacteriaceae is a major public health threat associated with significant morbidity and mortality, and mainly due to Klebsiella pneumoniae and Escherichia coli. However, there is little information about carbapenemase-producing (CP) Klebsiella oxytoca, an important nosocomial pathogen. We characterized CP K. oxytoca isolates collected from different Spanish hospitals between January…

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Posaconazole Salvage Therapy: The Posifi Study.

PCZ salvage therapy, especially oral tablets, can be an effective alternative option for patients with IFI who cannot tolerate or do not respond to other antifungal therapies. This is the result of a collaborative multicentre study carried out in six tertiary hospitals in Spain to evaluate the use of PCZ in different forms of administration…

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Usefulnesss of guideline recommendations for prognosis in patients with candidemia.

Last December the journal “Medical Mycology” published a multicenter study of adult patients with candidemia in 13 hospitals in Spain, lead by investigators of Bellvitge University Hospital. Candidemia is a common infection and is associated with high morbidity and mortality. Outcomes of patients with candidemia may be directly influenced by the general management, including diagnostic…

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Understanding and modelling reservoirs, vehicles and transmission of ESBL-producing Enterobacteriaceae in the community and long-term care facilities: the MODERN project

MODERN is an international collaborative project investigating the reservoirs and transmission of Enterobacteriaceae producing extended-spectrum β-lactamases (ESBL-E), funded by national institutions participating in the Joint Programme Initiative – Antimicrobial Resistance (JPI AMR) of the European Union with 1 million Euros. The project is coordinated by Hospital Universitario Virgen Macarena/University of Seville/Institute of Biomedicine of Seville,…

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Scheduled monitoring of host’s immune responses is useful to prevent the development of opportunistic infections after kidney transplantation.

A recently published multicenter study led by Dr. Mario Fernández-Ruiz and Prof. José María Aguado, from the University Hospital “12 de Octubre” (Madrid), has confirmed the usefulness of a immune-based monitoring strategy to predict the risk of potentially severe opportunistic infections due to the reactivation of cytomegalovirus (CMV) in patients undergoing kidney transplantation. The study…

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Antibiotic-induced genetic variation: how it arises and how it can be prevented.

In a recent publication (Annual Reviews of Microbiology), scientists belonging to REIPI (Centro Nacional de Biotecnología, Hospital Virgen del Rocío and Hospital Ramón y Cajal), in collaboration with the Université París Descartes-CNRS, have called the attention on the dangerous side effects of antibiotics because of their capacity to accelerate the rise of antibiotic resistant bacteria.…

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Synergistic activity of an OmpA inhibitor and colistin against colistin-resistant Acinetobacter baumannii: mechanistic analysis and in vivo efficacy.

“The sharp increase in cases of MDR Acinetobacter baumannii infections in recent years prompted by WHO to list this pathogen as a critical pathogen. The development of new approaches that adjuvant the scarcity of clinically relevant antibiotics for treatment of infections by MDR A. baumannii is an urgent need. Researchers from the University Hospital Virgen…

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Suppression of the SOS response modifies spatiotemporal evolution, post-antibiotic effect, bacterial fitness and biofilm formation in quinolone-resistant Escherichia coli.

Journal of Antimicrobial Chemotherapy 2018 (In press)   This study was coordinated at University Hospital Virgen Macarena in collaboration with the Centro Nacional de Biotecnología. This work shows that suppression of the SOS response (through recA deleted) blocks the evolution towards clinical quinolone resistance across a range of E. coli phenotypes from highly susceptible to…

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Prospective Surveillance Triazole Resistance fungal in Spain (FILPOP2 study)

Antifungal resistance is increasing by the emergence of intrinsically resistant species and by the development of secondary resistance in susceptible species. A previous study performed in Spain revealed levels of azole resistance in molds between 10 to 12.7% but secondary resistance in A. fumigatus was not detected. We used itraconazole supplemented media to select resistant…

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Analysis of intestinal colonization by ESBL-E. coli reveals the emergence in Europe of CTX-M-27 associated with clade C1-M27 of ST131 clone

– This finding represents an epidemiological change in extended-expectrum-beta-lactamases (ESBLs) distribution in Europe – This study results from the collaboration of the Preventive Medicine and Microbiology Departments at Hospital Universitario Ramón y Cajal (Madrid) with a European multicentre project (R-GNOSIS) funded by the EU and it has been recently awarded as best communication in the…

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